In this study, nondried solid residues were used as a substrate for enzymatic hydrolysis after pre-treatments to favour the disruption of inter- and intrachain hydrogen bonding of cellulose fibrils. This leads to a decrease in the crystallinity of cellulose and positively affects the cellulose swelling, thus enhancing enzymatic digestibility of lignocellulosic biomass (Luo and Zhu, 2011). The results of the whole saccharification process in which physical