Wild mushrooms are important sources of nutrients and bioactive compounds, namely phenolic acids.
After their ingestion, bioactive molecules have to be released from the matrix to be absorbed by the
organism. In the present work, two wild edible mushrooms (Volvopluteus gloiocephalus and Clitocybe
subconnexa) were studied for their nutritional value, detailed chemical composition and antimicrobial
activity. Bioaccessibility studies were also performed using the in vitro digestion of the crude powder,
phenolic extracts and individual phenolic acids identified in the samples. The studied species proved to
be rich sources of nutrients, minerals and bioactive molecules such as phenolic acids. The in vitro
digestion conducted to a decrease in antibacterial activity, but not in antifungal and demelanizing
properties. Nevertheless, in most of the cases, the analyzed samples presented higher antibacterial and
antifungal activities than the standards. The bioactive molecules (phenolic acids) were found in higher
concentrations in the phenolic extracts before in vitro digestion, which is agreement with the highest
antibacterial activity revealed by these extracts. It should be highlighted that the phenolic acids were still
bioavailable after digestion.