Determination of analytical sensitivity and specificity
To determine analytical sensitivity, non-infected N. aperta or O. nosophora snails were crushed separately. Subsequently, individual aliquots of 1, 5 (pooled), and 10 (pooled) non-infected N. aperta and O. nosophora snail samples were each separately inoculated with 1, 5, and 10 S. mekongi and S. japonicum cercariae.