According to the co-culture experiment, L. plantarumMRO3.12 completely inhibited V. harveyi under both aerobic and anaerobicconditions, meanwhile no reduction of L. plantarumMRO3.12 wasobserved. The dramatic drop of V. harveyioccurred after 12 h of incubation (Fig. 1), at which L. plantarumMRO3.12 began to produce the antibacterial compound as appeared in Fig. 2. Under anaerobic condition, V. harveyi inhibition at 18 h was lower than
that occurred under aerobic condition. This phenomenon supported the previous result that hydrogen peroxide was a major agent contributing to the antibacterial effect. As it is well known
that LAB are able to generate hydrogen peroxide through the action of flavoprotein-containing oxidases, and superoxide dismutase inthe presence of oxygen [18]