Moreover the phytochemical characterization of the extract was also measured by determining total phenolic, flavonoids, tannin and ascorbic acid contents. The characterization of the extract was also done by HPLC profiling with standard gallic acid. The present study demonstrated that extract have significant reducing capacity and scavenging of nitric oxide as well as hydrogen peroxide induced radicals. This activity of the extract may be due to the total polyphenolic contents present in it. The antioxidant activity of the extract was more significant than standard ascorbic acid in a concentration-dependent manner. The present study concluded that a polyphenolic rich fraction of T. chebula fruits is a potential source of natural antioxidants