The collected samples were analyzed for pH, acidity, specific gravity, protein, antioxidant activity (DPPH assay), phenolics, anthocyanins, colour intensity and melting property. The pH
wasmeasured by dissolving 1 g of ice creamsample in 9 ml of distilled water. For estimation of acidity, 10 g of ice cream sample was diluted with 10 ml of water. After addition of 3–4 drops of Phenolphthalein indicator titration was carried out with 0.1 N NaOH (Margaret et al. 2007). Frozen ice cream samples were thawed out in a refrigerator at 4 °C for 24 h, and then it was kept at 20 °C. The melted ice cream was put into a cylinder, and the volume and weight were recorded to calculate the specific weight of ice-cream samples (Winton 1958). The melting property of ice cream samples were calculated as per method suggested by Martin-Voulasiki and Zerfirdis (1990). The samples having 50 ml of hardened ice cream were allowed to melt on wire mesh at room temperature. The material that melted and drained through the wire mesh was
collected and weighed every 10 min. Phenolic content of samples was determined with
Folin-Ciocalteu reagent by