The 118 field samples were analyzed using the quadruplex RT-PCR assay and the ICS. Of the 118 samples, 41 positives and 77 negatives were obtained with the ICS, versus 40 positives and 78 negatives by means of quadruplex RT-PCR (Table 1). Thus,compared with the quadruplex RT-PCR assay, the ICS test had a specificity of 98.7%, and a sensitivity of 100%. There was excellent agreement ( = 0.982) between the ICS results and the quadruplexRT-PCR results