Partial actin (ACT), β-tubulin (TUB2), calmodulin (CAL), glutamine synthetase (GS), glyceraldehyde-3-phosphate dehydrogenase (G PDH) genes and the complete rDNA-ITS (ITS) region from 15 representative Colletotrichum strains were amplified by PCR reaction. The same strains of new species were used in the pathogenicity tests