Vitamin D plays an important role in calcium metabolism and affects other metabolic pathways. Despite
the intense interest in vitamin D, no comprehensive overview addressing the analysis of vitamin D in
milk has been published.
Historically, immunoassay techniques have been mainly used for the routine quantification of vitamin
D and its metabolites. However, the greater accuracy and precision of chromatography makes it one of
the most important methods in the analysis of vitamin D. The determination of vitamin D and its metabolites
by LC–MS is the gold standard for its assessment. LC–MS has unique advantages for vitamin D
determination and quantification due to its high sensitivity and specificity.
In this review, the current status of vitamin D and its metabolites analysis in milk, human and bovine,
including sample pre-treatment and chromatography analysis, are critically discussed and summarised.
Vitamin D plays an important role in calcium metabolism and affects other metabolic pathways. Despite
the intense interest in vitamin D, no comprehensive overview addressing the analysis of vitamin D in
milk has been published.
Historically, immunoassay techniques have been mainly used for the routine quantification of vitamin
D and its metabolites. However, the greater accuracy and precision of chromatography makes it one of
the most important methods in the analysis of vitamin D. The determination of vitamin D and its metabolites
by LC–MS is the gold standard for its assessment. LC–MS has unique advantages for vitamin D
determination and quantification due to its high sensitivity and specificity.
In this review, the current status of vitamin D and its metabolites analysis in milk, human and bovine,
including sample pre-treatment and chromatography analysis, are critically discussed and summarised.
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