Rooting of regenerated shoots
Results showed that there was a significant difference between two cultivars of carnation in rooting
percentage. So that the highest root percentage for Eskimo Mogr (83%) were achieved on the medium
supplemented with 0.5 mg/l NAA, while for Innove Orange Bogr (98%) it was obtained on the medium
containing 1.5 mg/l IBA. These findings confirmed the different reactions of various cultivars of carnation to
different kinds of auxins. In addition, plant growth regulators significantly influenced the height and number
of produced roots. Generally, the highest root number and root length was occurred at the range of 1.5-2.5
mg/l of NAA or IBA but the best rooting percentage was achieved at the range of 0.5-1.5 mg/l of these two
growth regulators. By increasing the concentration of auxin up to 0.5 mg/l, rooting percentage increased. But
generally higher concentrations of auxin led to decreasing the rooting percentage (Table 2).
Auxin plays a major role in root induction through its effect on the first cell division which forms root
initials (Farooq et al. 2008). Salehi (2008) found that there is a great deal of variation among cultivars of
carnation in their requirements for plant growth regulators in vitro and rooting of carnation cultivars is highly
genotype dependent. He reported that recommending the general medium for rooting of different carnation
cultivars, is not possible. Results of his study showed that plantlet with higher root numbers have the lower
root lengths. But our results did not show this negative relation. Ali et al. (2008) found that among the
various concentrations of NAA and IBA, the best rooting response were obtained on MS medium containing
1 mg/l NAA. Also, there are several reports on suitability of auxin- free medium for rooting of carnation
cultivars (Mii et al. 1990, IIahi et al. 1995). Notwithstanding these reports, we observed that auxin- free
medium did not result in a good rooting percentage, may be due to genotype differences. These results are in
accordance with the finding of Cuzzuol et al. (1996) and Jagannatha et al. (2001).
รากของหน่ออาศัยผลการศึกษาพบว่ามีความแตกต่างที่สำคัญระหว่างสองสายพันธุ์ของดอกคาร์เนชั่นในรากร้อยละ เพื่อให้รากร้อยละที่สูงที่สุดสำหรับเอสกิโม กำลังประสบความสำเร็จในการสื่อเสริมด้วย0.5 มันได้รับในสื่อที่มี1.5 mg / l IBA Rooting of regenerated shoots
Results showed that there was a significant difference between two cultivars of carnation in rooting
percentage. So that the highest root percentage for Eskimo Mogr (83%) were achieved on the medium
supplemented with 0.5 mg/l NAA, while for Innove Orange Bogr (98%) it was obtained on the medium
containing 1.5 mg/l IBA. These findings confirmed the different reactions of various cultivars of carnation to
different kinds of auxins. In addition, plant growth regulators significantly influenced the height and number
of produced roots. Generally, the highest root number and root length was occurred at the range of 1.5-2.5
mg/l of NAA or IBA but the best rooting percentage was achieved at the range of 0.5-1.5 mg/l of these two
growth regulators. By increasing the concentration of auxin up to 0.5 mg/l, rooting percentage increased. But
generally higher concentrations of auxin led to decreasing the rooting percentage (Table 2).
Auxin plays a major role in root induction through its effect on the first cell division which forms root
initials (Farooq et al. 2008). Salehi (2008) found that there is a great deal of variation among cultivars of
carnation in their requirements for plant growth regulators in vitro and rooting of carnation cultivars is highly
genotype dependent. He reported that recommending the general medium for rooting of different carnation
cultivars, is not possible. Results of his study showed that plantlet with higher root numbers have the lower
root lengths. But our results did not show this negative relation. Ali et al. (2008) found that among the
various concentrations of NAA and IBA, the best rooting response were obtained on MS medium containing
1 mg/l NAA. Also, there are several reports on suitability of auxin- free medium for rooting of carnation
cultivars (Mii et al. 1990, IIahi et al. 1995). Notwithstanding these reports, we observed that auxin- free
medium did not result in a good rooting percentage, may be due to genotype differences. These results are in
accordance with the finding of Cuzzuol et al. (1996) and Jagannatha et al. (2001).
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