At first, Anderson medium for shoot induction and multiplication was used. In the experiment, ½ Anderson
basal medium with 0.01 mg/l indol acetic acid (IAA) were used in total of the treatments. All of the medium in the
current work were supplemented with 30 gr sucrose and 7 gr Difco-Bacto agar (sigma A-1296) and the pH was
adjusted to 5.8 with 1 N NaOH or 0.1 N HCl prior to autoclaving at 121°c for 30 min.