For immunization with rLm-gp33 or rLm-np118, mice
were inoculated intraperitoneally (i.p.) with 104 CFU of rLm in 0.5 ml of phosphate-
buffered saline (PBS). For i.c. inoculation of virus, mice were anesthetized
with isoflurane and the amount of virus designated in each experiment was
diluted in PBS containing 0.75% bovine serum albumin, and a total volume of 20
l was injected into the left cerebral hemisphere. For i.p. inoculation, virus in
PBS containing 0.75% bovine serum albumin, and a total volume of 500 l was
injected. Mock-infected controls were inoculated similarly but with an uninfected
cell lysate at a comparable dilution.