A total of 129 milk samples were collected from dairy farms in three distinct periods (dry period, transition period and rainy period), and analyzed by enzyme-linked immunoabsorbent assay (ELISA) as screening test. Samples with AFM1 at concentrations above 0.05 mg/L were analyzed by liquid chromatography with fluorescence detection (HPLC-FD) as confirmatory method. All the analyzed samples showed contamination with AFM1. In the three periods, AFM1 was detected at concentrations below the permitted limit of 0.50 mg/L in milk, according to the Brazilian legislation, and 18 samples
(13.95%) showed contamination with AFM1 above the permitted limit of 0.05 mg/L established by Codex Alimentarius and European Commission.