2.6. Chemical injury to erythrocytes
To assess any chemical injury to erythrocytes that might be attributed to the extract, 200 毺L of erythrocytes were incubated with 100 毺g/mL of the extract at a dose equal to the highest used in the antiplasmodial assay.
The conditions of the experiment were maintained as in the case of antiplasmodial assay.
After 48 h of incubation, thin blood smears were stained with Giemsa stain and observed for morphological changes under high-power light microscopy.
The morphological findings were compared with those in erythrocytes that were uninfected and not exposed to extract[13].