Results represent mean ± SD of six replicated experiments. Data were recorded after 30
days of culture.
About 80 per cent of plantlets survival were observed after hardening of the
regenerated E. travancoricum for three weeks. Seventy per cent of the plants
transferred to pots survived and resumed growth (Fig. 1H). There was no
detectable phenotypic variation among the acclimatized plants. In conclusion,
the results showed the ability of the shoot tip explants to produce 29 shootlets
without any intervening callus phase, where all the plantlets were uniform in
height and growth. As no reports are available on regeneration of E.
travancoricum from shoot tip explants, it is proposed this protocol for the first
time to obtain more plantlets within a short period.