The PCR product was purified by using
Montage PCR Clean up kit (Millipore) in order to remove unincorporated
PCR primers and dNTPs from PCR products. The purified
PCR products were sequenced by using 2 primers, 518F0 (50-
CCAGCAGCCGCGGTAATACG-30) and 800R0 (50-TACCAGGGTATCTAATCC-
30).