functional library screening must tightly couple the observable phenotype to the encoding
genotype. In this case the desired phenotype is enzyme mediated bacterial killing in the presence of lvyc, and the cognate genotype is encoded by the individual killing is readily
quantified using various vaibility probes coupling bacterium death to the causative genotype of a separate expression host represent a technically challenging problem in
ultra-high throughput formats. To identify hlyz variants able to kill bacteria in the presence of Ivyc, we modified our previously.