The pathogenic fungus,M. eumusaewas isolated fromthe leaf spot
affected samples collected from cv. Grand Naine using potato dextrose
agar (PDA) medium using the spore pick method under laboratory
conditions. The culture plates were maintained at 25 C for
seven days. Mycelial culture from the plate was then transferred to
PDA slants andmaintained at 25 C (Ainsworth,1971) till further use