Grape berries, especially the interface between soluble nutrients
and the septic world, are common niches for yeasts.
From a biotechnological point of view, grapes are the primordial
source of the microorganisms necessary for alcoholic fermentation
to occur, providing must with both beneficial and
potentially spoilage species. Nevertheless, the yeast flora of
grapes is surprisingly poorly documented (13, 19). As determined
so far, the physiognomy of the grape microflora may
change in response to factors such as the climate, grape variety,
and geographical region (4, 18, 21). Biological invasion is a
critical concern for widespread changes in the community.
Botrytis is among the most important pathogens that cause
grape damage (gray rot) or drying (noble rot), yet its role in
yeast ecology has not been studied previously. We assessed and
compared yeasts present on Botrytis-infected and healthy
grapes. Different molecular methods were used for species
identification, and the robustness of these methods is discussed
below.
Grape berries, especially the interface between soluble nutrients
and the septic world, are common niches for yeasts.
From a biotechnological point of view, grapes are the primordial
source of the microorganisms necessary for alcoholic fermentation
to occur, providing must with both beneficial and
potentially spoilage species. Nevertheless, the yeast flora of
grapes is surprisingly poorly documented (13, 19). As determined
so far, the physiognomy of the grape microflora may
change in response to factors such as the climate, grape variety,
and geographical region (4, 18, 21). Biological invasion is a
critical concern for widespread changes in the community.
Botrytis is among the most important pathogens that cause
grape damage (gray rot) or drying (noble rot), yet its role in
yeast ecology has not been studied previously. We assessed and
compared yeasts present on Botrytis-infected and healthy
grapes. Different molecular methods were used for species
identification, and the robustness of these methods is discussed
below.
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