An abundant 100 kDa-band was selected for use in MADLI TOF
MS identification. Previous work [12] identified a similar sized band
in staphylococcal species isolated from indoor air as aconitate
hydratase. The identification of a unique aconitate hydratase
sequence in Micrococcus could provide an additional means for the
unequivocal identification of the two genera without the need for
physiological tests. Batches of protein extracts from environmental
strains were analyzed, together with two ATCC M. luteus reference
strains (ATCC 4698 [the most frequently used reference standard]
and ATCC 49732). S. warneri (ATCC 49454), S. aureus (ATCC 31240)
and S. hominis (ATCC 27844) were used as additional control strains.