2.4.2. Cellulase production
The standardized protocols of Hendricks et al. (1995) were used
to evaluate the cellulase production. Actinomycetes were streaked
on cellulose Congo red agar media and incubated at 28 2 C for
four days. The plates were observed for halo zone around the
actinomycete colonies, which indicate the presence of cellulase.
Observations were recorded on a 04 rating scale as follows: 0 ¼ no
change; 1 ¼ positive; 2 ¼ halo zone of 1e3 mm; 3 ¼ halo zone of
4e6 mm and 4 ¼ halo zone of 7 mm and above.