Permeation Studies and Application of
Penetration Enhancers (PEs)
Permeation experiments were carried out using
modified Franz diffusion cells at 32 °C. Prior to
treatment with PEs hoof membranes and keratin films
were hydrated for one hour with PBS and were then
mounted between the compartments (donor &
receiver). The receiver was filled with PBS and the PE
solution was filled into the donor compartment.