The organisms present in the soil were screened for their antibiotic production potential by crowded plate technique 23 Nutrient agar was used in place of Tripticase soy agar). Twelve) .bacteria that showed promising activity were selected, isolated in pure form and preserved at 4 oC. Among the twelve, the two bacteria KBAIA-0210 and JKNAB-0609 which showed very good antibacterial activity against all the target organisms were selected for identification and further screening for other pharmacological activities.