and filling two
heparinized microhematocrit tubes from the exposed caudal vein. The tubes were centrifuged
for 10 min at 4000 X g, and hematocrits were measured. A portion of the remaining
blood was used for direct counting of red blood cells and white blood cells. The blood was
diluted 1: 100 with Yokoyama’s solution (Yokoyama, 1960)