Enrichment method
Test samples were weighed (25 g) and transferred aseptically to
a presterilized conical flask containing 225 mL of the alkaline
peptone water with 3% NaCl and kept in the temperature controlled
horizontal bench shaking incubator (Orbitek, India) for about
10e15 min. After 24 h duration 1 mL of inoculum was transferred to
a sterile petridish and plated by using the selective media such as
TCBS (Thiosulfate Citrate Bile Salt Sucrose Agar). The plates were
incubated at 37 C for 24 h. Isolated colonies with differences in
morphological features were transferred into nutrient agar slants