The objective of this study was to gain information about the toxigenic Aspergillus species present in
a wide survey of retail samples of paprika and chilli collected in Spain. Detection of these mycotoxigenic
species was performed with an optimized protocol for paprika and chilli which includes a set of speciesspecific
PCR assays. Occurrence of toxigenic Aspergillus species was higher in paprika than in chilli
samples (83.9% and 64.5%, respectively). Paprika showed also the highest percentage of co-occurrence of
two or more different species (43.6%) in comparison with chilli (35.5%). The most common aspergilli
were Aspergillus niger aggregate (67.7%), followed by Aspergillus flavus (49.5%). Aspergillus carbonarius,
Aspergillus parasiticus and Aspergillus steynii were detected at lower frequency (1.1%). The high cooccurrence
of Aspergillus species able to produce ochratoxin A and aflatoxins, particularly in paprika,
suggested the need of a more efficient control during processing and storage to reduce fungal
contamination, and additional legislation to consider the simultaneous presence of both toxins in these
matrices.
The objective of this study was to gain information about the toxigenic Aspergillus species present ina wide survey of retail samples of paprika and chilli collected in Spain. Detection of these mycotoxigenicspecies was performed with an optimized protocol for paprika and chilli which includes a set of speciesspecificPCR assays. Occurrence of toxigenic Aspergillus species was higher in paprika than in chillisamples (83.9% and 64.5%, respectively). Paprika showed also the highest percentage of co-occurrence oftwo or more different species (43.6%) in comparison with chilli (35.5%). The most common aspergilliwere Aspergillus niger aggregate (67.7%), followed by Aspergillus flavus (49.5%). Aspergillus carbonarius,Aspergillus parasiticus and Aspergillus steynii were detected at lower frequency (1.1%). The high cooccurrenceof Aspergillus species able to produce ochratoxin A and aflatoxins, particularly in paprika,suggested the need of a more efficient control during processing and storage to reduce fungalcontamination, and additional legislation to consider the simultaneous presence of both toxins in thesematrices.
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