can be present in food and cosmetics and is colorimetrically detected by Folin-Ciocalteau reagent [45].
Therefore, the separation of ascorbic acid from ferulic acid before detection is required. The separation
of ascorbic acid and ferulic acid by TLC was performed with a 85:15:1 (v/v/v) chloroform: methanol:
formic acid mobile phase (Figure S6), where the two analytes could be clearly separated with Rf values
of 0.70 ± 0.01 and 0.10 ± 0.01 for ferulic acid and ascorbic acid, respectively