Odontophrynus americanus
The animals (5 females and 10 males) were collected at
night in late February and early March 2009 during an
evening spawning event in the vicinity of Verónica, Buenos
Aires province, Argentina (35°28'34''S, 57°09'30''W).
They were transported and housed for 30 days prior to
experimentation in terraria at UNLP. Two trials were performed
with this species. For trial 1, one female and 2
males were placed in each of 3 terraria consisting in fiberglass
tanks of 0.7 m2 containing earth and water simulating
their natural habitat. All animals were injected with
AMPHIPLEX on March 16, 2009 in the morning. For
trial two, adult frogs were collected in March 2009 and
kept in captivity as described for a period of approximately
4 months. One female and 2 males were placed in
each of 5 terraria and injected with AMPHIPLEX on
August 4, 2009. On average, females weighed 9.0 ± 1.0 g
and males 8.5 ± 0.4 g.
For all the three species, once the fertilized eggs were
obtained, they were incubated at 25 ± 1°C, phoperiod
16L:8D, pH 7.6-8.3, with aeration. They were reared in
different aquaria at 10 individuals per liter until metamorphosis.