Spectrometric analysis was conducted according to
the method described by Law and Slepecky (1960). The extracted
PHB was dissolved in 10 ml of sulfuric acid (36 N), and the resultant
solution was heated at 100 C on a water bath for 10 min.
Addition of sulfuric acid converts the PHB to crotonic acid. The
solution was cooled and the absorbance was measured at
235 nm for determining the PHB concentration against a sulfuric
acid blank. Standard curve was prepared using pure PHB