chromatographic performed at 25 °C with a flow rate
of 0.8 mL/min. Binary mobile phase was prepared according to the reported
method (Sun, Cao, Bai, Liao, & Hu, 2010). Solvent A was water
and formic acid in a ratio of 99:1 (v/v), respectively. Solvent B was
water, methanol and formic acid in a ratio of 50:49:1 (v/v/v). The
gradient system was 40% solvent B in the first 2 min, 100% solvent B at
42 min, and 40% solvent B at 47 min. C3G concentrationwas determined
according to the standard curve.