The AOAC and ISO methods require large organic solvent volumes
to extract the lipids as compared with the Folch method.
These solvent volumes can directly influence the cost of the analyses.
Estimated costs of extraction alone are $3.7, $2.5, and $3.7 US
per sample for the AOAC, ISO and Folch methods, respectively.
Reducing solvent volumes without compromising analytical
outcomes would reduce the financial cost of monitoring the food