Bovine oviductal epithelial cells (four replicates) were
mechanically isolated, pooled, and cultured as conventional monolayers, as explants, and in a
polarized cell culture system with Dulbecco’s modified Eagle’s medium/F12–based culture
medium. Bovine oviductal epithelial cells were exposed to an NEFA mixture of oleic, stearic,
and palmitic acids for 24 hours at both physiological and pathologic concentrations. A control
(0 mM NEFA) and a solvent control (0 mM NEFA þ 0.45% ethanol) group were implemented.