The effects of various parameters such as reagent composition, temperature, volume of the injection loop, and flow rate on the determination process were investigated and optimized via un ivariate experimental design.
It should be noted that the length of the MC was chosen as 350 cm with out optimization, merely for the ease of immersing the knotted coil in the water bath.
A 20 mM nitrate standard solution was used throughout the parameter-optimization procedures