except for an annealing temperature of 59 °C. The presence and size of
the amplified DNA product was determined using a 3% agarose gel. To
avoid DNA contamination issues and false positive results, the methods
of DNA isolation, PCR, and electrophoresis were performed in separate
rooms using different pipettes, racks, filter tips, and gloves. The PCR
mixes were prepared in a sterile PCR box, and all tests in this study included
positive and negative controls. All positive samples tested