The PCR products
of phaC2 were digested with XhoI/KpnI and inserted
into XhoI/KpnI-hydrolyzed pBlueC1, resulting in plasmid
pBlueC1C2. A 3,624-bp DNA fragment containing the
phaC1 and phaC2 genes was amplified from pBlueC1C2 by
PCR employing Pfu DNA polymerase and a pair of primers
amC1-F/C2-R1.