Based on the screening tests, the inhibitory spectrum of potential
bacteriocin-producing isolates was assessed against 42 indicator
strains using a standardized agar disc diffusion test.
Accordingly, an aliquot of 20 ml CFS was applied on sterile filter
paper discs (6 mm diameter) and set on agar plates previously
inoculated (0.1 ml) with each individual indicator strain suspension
corresponding a cell density of approx. 105 CFU/ml. Plates then
were incubated 24 h at optimum temperatures of the test organisms
(Table 1). Antimicrobial activity was detected as a translucent
halo in the bacterial lawn surrounding the discs.