Conclusions
In the present study, a new and simple HPLC method for
selective and sensitive simultaneous determination of neopterin,
kynurenine, tryptophan and creatinine in human serum using a
monolithic technology for clinical monitoring was developed and
validated.
The main advantage of this method is the combination of two
different size monoliths, for preliminary and analytical separation
with peak shape improvement and using of flow rate switching with low back pressure resulting in short time of analysis. Very
simple sample preparation using micro titration plates that allow
handling 96 samples during one extraction step. This method is
suitable for routine analyses (low price, small volume of samples
and solvents) of large sequences of samples which have to be measured
in a short time.