DNA extraction
DNA was isolated from ethanol-preserved tissue using the
REALPURE DNA extraction kit (REAL laboratories, Spain). Some
modifications were made to the manufacturer's instructions in order
to work efficiently with 96 well PCR (Polymerase Chain Reactions)
plates.Up to two fin biopsy punches (approximately 0.5 cm2 of fin tissue
in total) or fry tails were digested by 3 μl of proteinase K (10 mg/ml)
in the presence of 75 μl cell lysis solution during overnight incubation