The FAME were separated and quantified by gas-liquid
chromatography (Carlo Erba Vega 8160, Milan, Italy)
using a 30 m × 0.32 mm i.d. capillary column (CP
Wax 52CB, Chrompak, London, UK) and on-column
injection. Hydrogen was used as the carrier gas and
temperature programming was from 50°C to 150°C at
40°C/min and then to 230°C at 2.0°C/min. Methyl esters
were identified and quantified as described previously
(Kennedy et al., 2005). Individual FAME were
expressed as a percentage of all peaks.