2.6. Release from TiO2 nanotubes on Ti thin film
In order to investigate the release kinetic of the drug from the TiO2
nanotube layer on a Ti thin film, the specimen was immersed in
800 μl of PBS in a 4-well plate at room temperature in a shaking incubator
at 100 rpm. After a specified time, 500 μl of the sample was
collected to evaluate the amount of drug released. The collected solution
was changed with 500 μl of fresh solution every time to calculate
the accumulated sample concentration. The collected samples were
analyzed for drug concentration using the UV–VIS spectrophotometer
(UV–VIS, UVD-3200, Labomed, USA).