PGPR research on ISR in plants has mainly focused on fluorescent pseudomonad based PGPR strains with little emphasis on Bacillus PGPR ( Kloepper et al., 2004). Commercialization of fluorescent pseudomonad based products generally failed due to lack of long-term viability of these asporogenous bacteria. Gram-positive bacteria by virtue of their ability to form heat and desiccation-resistant spores can be formulated readily into stable products. Further, the use of rhizobacterial strain mixtures has its own advantages over individual strains in plant disease management. In this study, the Bacillus strain mixture S2BC-1+GIBC-Jamog was superior to individual strains and other mixtures in eliciting protection against vascular wilt disease both locally and systemically under greenhouse conditions. This mixture is now being tested in field trials to determine efficacy and achieving the goal of practical application of ISR in the field.