Uroplatus geckos are exclusively nocturnal. The larger species spend most of the daylight hours hanging vertically on tree trunks, head down, resting, while the smaller leaf tailed geckos (U. phantasticus, U. ebenaui, U. finiavana and U. malama) spend more time in bushes and small trees imitating twigs and leaves. During the night, they will venture from their daytime resting spots, and go off in search of prey. They are all insectivores.
During their breeding season, female Uroplatus lay from 2-4 eggs depending on species and conditions.