Radical-scavenging activity (RSA) was assessed using the 2,2-
diphenyl-1-picrylhydrazyl (DPPH) assay (Miliauskas et al., 2004).
Different dilutions of extracts (1 mL) were added to 2 mL of DPPH (5.9 mg per 100 mL methanol). After 30 min, absorbance was
measured at 517 nm. RSA, expressed as ascorbic acid equivalent
antioxidant capacity (AEAC) in mg ascorbic acid/100 g, was
calculated as IC50(ascorbate)/IC50(sample) 105. IC50 of ascorbic acid
was 0.00387 mg/mL.