The fact that the
plant species simply die, if they come in touch with this raw wastewater sample signifying excessive toxic effect of the
ingredients of the wastewater to the plant, necessitates dilution of the anaerobically treated effluent 40 and 80 times by
mixing municipal wastewater with it in the ratio 1:39 and 1:79 respectively, These dilutions are selected after some trial
experiments ensuring sustenance of the aquatic macrophytes (Pistia stratiotes) meant for phytoremediation study, which
has been conducted for 10 days.