1. ABSTRACT: Catheter-related infections (CRIs) are associated
with the formation of pathogenic biofilms on the surfaces
of silicone catheters, which are ubiquitous in medicine.
2. These biofilms provide protection against antimicrobial agents and
facilitate the development of bacterial resistance to antibiotics.
3. The application of photothermal agents on catheter surfaces is
an innovative approach to overcoming biofilm-generated CRIs.
4. Gold nanoshells (AuNSs) represent a promising photothermal
tool, because they can be used to generate heat upon exposure
to near-infrared (NIR) radiation, are biologically inert at physiological temperatures, and can be engineered for the photothermal ablation of cells and tissue.
5. In this study, AuNSs functionalized with carboxylate-terminated organosulfur ligands were attached to model catheter surfaces and tested for their effectiveness at killing adhered Enterococcus faecalis (E. faecalis) bacteria.
6. The morphology of the AuNSs was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), while the elemental composition was
characterized by energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS).
7. Furthermore, optical and photothermal properties were acquired by ultraviolet−visible (UV-vis) spectroscopy and thermographic imaging with an
infrared camera, respectively.
8. Bacterial survival studies on AuNS-modified surfaces irradiated with and without NIR light were evaluated using a colony-formation assay.
9. These studies demonstrated that AuNS-modified surfaces, when illuminated with NIR
light, can effectively kill E. faecalis on silicone surfaces.
1. ABSTRACT: Catheter-related infections (CRIs) are associatedwith the formation of pathogenic biofilms on the surfacesof silicone catheters, which are ubiquitous in medicine.2. These biofilms provide protection against antimicrobial agents andfacilitate the development of bacterial resistance to antibiotics.3. The application of photothermal agents on catheter surfaces isan innovative approach to overcoming biofilm-generated CRIs.4. Gold nanoshells (AuNSs) represent a promising photothermaltool, because they can be used to generate heat upon exposureto near-infrared (NIR) radiation, are biologically inert at physiological temperatures, and can be engineered for the photothermal ablation of cells and tissue.5. In this study, AuNSs functionalized with carboxylate-terminated organosulfur ligands were attached to model catheter surfaces and tested for their effectiveness at killing adhered Enterococcus faecalis (E. faecalis) bacteria.6. The morphology of the AuNSs was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), while the elemental composition wascharacterized by energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS).7. Furthermore, optical and photothermal properties were acquired by ultraviolet−visible (UV-vis) spectroscopy and thermographic imaging with aninfrared camera, respectively.8. Bacterial survival studies on AuNS-modified surfaces irradiated with and without NIR light were evaluated using a colony-formation assay. 9. These studies demonstrated that AuNS-modified surfaces, when illuminated with NIRlight, can effectively kill E. faecalis on silicone surfaces.
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