Laboratory experiments were conducted to evaluate the chemical composition of fruit wastes (pulp and peels) of Banana andMango in order to explore their potential application in bio-ethanol production. The proximate composition of banana fruitpulp was 76.63% moisture, 5.65% protein, 1.37% lipid, 19.75% ash and 0.632% starch. Similarly for mango, the proximatecomposition of fruit pulp was 81.26% moisture, 7.96% protein, 1.48% lipid, 13.08% ash and 0.507% starch. The total dietaryfibre content ranged from 3.54% to 73.04% in the fruit samples and found at higher level in mango peels. A maximumpolyphenol content of 54.45% was observed in mango fruit peels and a minimum of 10.97% was recorded in banana fruitpulp. The dilute acid (H2SO4) pretreatment (DAP) followed by enzymatic hydrolysis showed maximum reducing sugar yieldof 64.27% in the mixed fruit pulps, followed by the banana fruit pulp (57.58%). The banana fruit peels also yielded amaximum reducing sugar content of 36.67% where as the lowest of 31.29% was observed in mango fruit peels. Thefermentation of the DAP hydrolysate of mixed fruit pulps showed maximum ethanol production of 35.86% corresponding to afermentation efficiency of 70.31% at 48 hr of incubation. Similarly, the hydrolysates obtained from the dilute H2SO4pretreated banana fruit peels yielded a maximum of 13.84% ethanol with a fermentation efficiency of 27.13% at 42 h ofincubation. The present study revealed that the fermentation of hydrolysates obtained from the dilute acid pretreatmentfollowed by enzymatic saccharification of mixed fruit pulps (banana and mango) and the banana fruit peels were found to bebest for higher ethanol production at optimized conditions