Interferences may be caused by chemical-protein and/or chemical-dye
interactions. Table 1 lists those chemical reagents not directly affecting
the development of dye color. (Note: Basic buffer conditions and detergents
interfere with this assay.) Since every protein-chemical reagent
combination has not been assayed, it is possible that some of the listed
reagents produce interference in combination with certain proteins. However, with respect to proteins such as bovine serum albumin and gamma globulin, the listed reagents show little or no interference. The acceptable concentrations of reagents for the Standard Procedure are shown in Table 1. Equivalent concentrations of reagents for the Microassay Procedure (see Section 2) are 1/40 of those listed in this table, due to the difference of sample-to-dye ratios between the Standard and Microassay Procedures.