2.2. Chemicals
Chemicals were all of analytical grade. Media and chemicals
used for isolation and lipase assay like p-nitrophenyl palmitate
and tributyrin were of high grades and purchased from Sigma and
Hi-Media Laboratories, India. The oil (olive oil) used as a substrate
in the lipase assay medium containing peptone, yeast extract,
NaCl, MgSO4 was purchased from the local market.