For growth study, 2.5 ml of 24 h old culture prepared in nutrient broth was inoculated into a 25 ml nutrient broth flask and flasks containing different concentrations of Malathion, Methamidophos, Cartap and Cypermethrin. Non shaken condition was used as it actually represents the environmental conditions where microorganisms are usually exposed. A control experiment without insecticide in nutrient broth was used for comparison. Samples from each flask were drawn, tested and counted. Miles and Misra technique was used for bacterial growth study.
The growth of IES-Ps-1 in biosimulator was determined by viable cell enumeration immediately after inoculation and at 24, 48, 72, and 96 h later. However, to determine the IES-Ps-1 capability for Cypermethrin degradation, batch experiments were performed using the biosimulator (activated sludge) at different temperatures, dissolved oxygen and Cypermethrin concentration. Approximately, 350 ml bacterial culture inoculated into wastewater sample (8.5 L) containing an appropriate quantity of Cypermethrin was transferred into the borosilicate glass jar of a compact bench scale stainless steel biosimulator ( Fig. 1). The sample was strongly agitated by impeller with flat stirring paddles and by four vertical baffles. The required temperature was maintained by the built in thermostat and the DO concentration was achieved by diffused aeration using pressure pump and mechanical aeration regulated through continuous agitation
For growth study, 2.5 ml of 24 h old culture prepared in nutrient broth was inoculated into a 25 ml nutrient broth flask and flasks containing different concentrations of Malathion, Methamidophos, Cartap and Cypermethrin. Non shaken condition was used as it actually represents the environmental conditions where microorganisms are usually exposed. A control experiment without insecticide in nutrient broth was used for comparison. Samples from each flask were drawn, tested and counted. Miles and Misra technique was used for bacterial growth study.
The growth of IES-Ps-1 in biosimulator was determined by viable cell enumeration immediately after inoculation and at 24, 48, 72, and 96 h later. However, to determine the IES-Ps-1 capability for Cypermethrin degradation, batch experiments were performed using the biosimulator (activated sludge) at different temperatures, dissolved oxygen and Cypermethrin concentration. Approximately, 350 ml bacterial culture inoculated into wastewater sample (8.5 L) containing an appropriate quantity of Cypermethrin was transferred into the borosilicate glass jar of a compact bench scale stainless steel biosimulator ( Fig. 1). The sample was strongly agitated by impeller with flat stirring paddles and by four vertical baffles. The required temperature was maintained by the built in thermostat and the DO concentration was achieved by diffused aeration using pressure pump and mechanical aeration regulated through continuous agitation
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