e, which was assayed as shown by Iijima et al. (1998). Trypsin and pancreatic lipase were measured according to Rotllant et al. (2008), modified by Toledo-Cuevas et al. (2011). Total alkaline proteases were performed based on Twining (1984) and amylase activity was recorded by the EnzChek Ultra AmylaseAssay kit (E33651 Molecular Probes, Invitrogen). The fluorometric measurements were carried out using a Fluoroskan Ascent equipment (Thermo Fisher Scientific, Vantaa, Finland). The activities were reported as total activity [Fluorescence Units (FU) per segment (FU segment-1)] and specific activity (FU mg protein-1). For neutral lipase, 1 U of enzyme activity was defined as 1 mmol of hydrolyzed substrate released per min. Each assay was performed in duplicate. Protein concentration was measured in homogenates as described by Bradford (1976).